Laundry Detergent Testing Guidelines - Minimum requirements for comparative detergent testing

Laundry detergents are sometimes compared on performance, e.g. by consumer organisations. But to be useful for consumers, it is important that these tests are of high quality and offer a realistic and reliable comparison of product characteristics.

A.I.S.E. developed its first detergent testing guidelines in 2009 and has since been updating it on a regular base, making it available to manufacturers and other interested parties such as consumer organisations and testing institutes. 
The protocol provides a minimum requirement methodology for assessing the performance of laundry detergents (heavy duty and light duty) and laundry additives across Europe. The Guidelines have been developed based on experts' knowledge from major companies manufacturing such products, after a thorough review of existing test protocols used in Europe and of existing internal company data.


The protocol comprises (as minimum requirements - bearing in mind that organisations may feel free to exceed them): 

A.I.S.E. reviews this protocol regularly. Please address your feedback, reactions, suggestions and questions directly to

Status of the review of the protocol – May 2020

Stain Set - several changes to the stain set to improve consumer relevance and discriminative power. The stain set still comprises 14 stains and contains 3 new stains and 2 alternative supplier choices.

Dye Transfer Inhibition Protocol
- this project has been temporarily suspended for priority reasons.

Whiteness Maintenance Protocol
- the group has started a programme to further improve the whiteness test method in a more consumer relevant way. The screening tests with different approaches have been nearly completed by different test institutes. The group will take this on as the next project for 2020.

History of protocol update

 Version  Date  Comments
 V.1.  February  2009  Original stain set
 V.2.  July 2012  Changes to the stain set:
 Interim situation for the red wine stain, as offered by Equest (one  of the stain providers)
 V.3.  June 2013  Changes to the stain set:
 New stains for red wine and frying fat (hamburger grease)
 V.4.  July 2013  Changes to the stain set:
 Replacement of stain WFK 10M for unused motor oil by WFK  10RM
 V.5. November 2013  Changes to the stain set:
 New stains for coffee and tea
V.5.1. April 2016 No changes to the test requirements, only editorial changes.

The files have been updated to reflect change in the name of the project (previously "Detergent Test Protocol") and to include: initial disclaimer on the use of the protocol; translation from English of missing text on slide related to the evaluation of degree of stain removal; inclusion of list of acronyms at the end.
V.5.2 June 2018 Clarification on the purpose of the guidelines (for comparative performance testing of detergents, NOT washing conditions) and on the choice of washing cycle(s).
V.6. May 2020
Changes to the stain set:
Replacement of Baby Food by Salad Dressing Balsamico C-S-406;
Replacement of Burnt Beef by Cooked Beef Fat on polyester/cotton WE5BBPC2;
Replacement of Grass by Pure Grass C-S-07 and addition of Scrubbed Grass WE5SGWKC as alternative choice;
Addition of Face Make-up WE5FM2WKC as alternative choice.
If you wish more information about the stain update, please check this document.